Composite

Part:BBa_K3576001:Design

Designed by: Shan Dong   Group: iGEM20_ASTWS-China   (2020-06-22)


PETase expression system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 97
    Illegal NgoMIV site found at 123
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 267


Design Notes

For strong PETase expression, PETase was inserted into pET-21a(+) vector and we did codon optimization for successful E. coli expression.

Source

The sequence was based on BBa_K2013002

References