Composite
Part:BBa_K3576001:Design
Designed by: Shan Dong Group: iGEM20_ASTWS-China (2020-06-22)
PETase expression system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 97
Illegal NgoMIV site found at 123 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 267
Design Notes
For strong PETase expression, PETase was inserted into pET-21a(+) vector and we did codon optimization for successful E. coli expression.
Source
The sequence was based on BBa_K2013002